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M9490157.TXT
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1994-09-03
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Document 0157
DOCN M9490157
TI Evaluation of monoclonal antibodies to HIV-1 envelope by neutralization
and binding assays: an international collaboration.
DT 9411
AU D'Souza MP; Geyer SJ; Hanson CV; Hendry RM; Milman G; Division of AIDS,
National Institute of Allergy and Infectious; Diseases, National
Institutes of Health, Bethesda, MD 20892.
SO AIDS. 1994 Feb;8(2):169-81. Unique Identifier : AIDSLINE MED/94318200
AB OBJECTIVE: To characterize a purified panel of monoclonal antibodies
(MAb) to epitopes in HIV-1 envelope V3, CD4-binding region, C4 and gp41.
DESIGN: Neutralization and/or binding activity data were obtained from
21 laboratories on a coded panel consisting of seven human MAb, seven
mouse MAb, recombinant human CD4 immunoadhesin [CD4-immunoglobulin G
(IgG)], normal human and normal murine Ig. METHODS: Laboratories
performed a variety of neutralization assays and antigen binding assays
with HIVIIIB, HIVMN and other laboratory strains of HIV-1. RESULTS: For
a single MAb, there was up to a 10(3) range of neutralizing antibody
titers between laboratories. The range in titers appeared to depend on
the sensitivity of the neutralization assay. Two methods were used to
consolidate the data from all laboratories, the geometric mean titer
(GMT) and the median neutralizing titer (MNT). The panel of MAb were
also analyzed by a variety of assays that measure binding activity to
native or denatured epitopes. The relative binding activity of the MAb
did not appear to correlate with neutralizing activity. CONCLUSION:
Neutralization results from any single laboratory did not correlate with
the collective data. The relative potency (rank order) of the MAb in the
panel were equivalent when determined by GMT or MNT. These values may be
useful to individual laboratories for estimating the sensitivity of
their neutralization assays. The study also identified potential
reference reagents with which neutralizing activity could be compared.
DE Amino Acid Sequence Animal Antibodies, Monoclonal/*IMMUNOLOGY
Antigen-Antibody Reactions Antigenic
Determinants/CHEMISTRY/IMMUNOLOGY/METABOLISM Antigens, CD4/METABOLISM
Binding Sites Comparative Study CHO Cells Enzyme-Linked Immunosorbent
Assay Gene Products, env/IMMUNOLOGY/METABOLISM Hamsters Human HIV
Antibodies/*IMMUNOLOGY/METABOLISM HIV Antigens/*IMMUNOLOGY/METABOLISM
HIV Envelope Protein gp120/IMMUNOLOGY/METABOLISM HIV Envelope Protein
gp41/IMMUNOLOGY/METABOLISM HIV-1/*IMMUNOLOGY International Cooperation
Mice Molecular Sequence Data Neutralization Tests Peptide
Fragments/IMMUNOLOGY/METABOLISM Protein Binding Protein
Precursors/IMMUNOLOGY/METABOLISM Recombinant
Proteins/IMMUNOLOGY/METABOLISM Reference Standards Reproducibility of
Results Saccharomyces cerevisiae Sensitivity and Specificity Support,
Non-U.S. Gov't Support, U.S. Gov't, P.H.S. JOURNAL ARTICLE
MULTICENTER STUDY
SOURCE: National Library of Medicine. NOTICE: This material may be
protected by Copyright Law (Title 17, U.S.Code).